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Part:BBa_K598025:Design

Designed by: Yan GONG, Xiaowei YAN   Group: iGEM11_Peking_R   (2011-09-28)


Bistable Switch with TPP Hammerhead Ribozyme 2.5 and Theophyline Riboswitch N8-3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1709
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2870


Design Notes

We utilize down-regulating TPP ribozyme TPP-1.20 and theophylline ribozyme Theo N8-3 affecting the green-red ratio of bistable switch in order to test our model.


Source

We construct this part by site mutation with the part BBa_K598024.

References